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Figure 5

From: Absence of Global Stress Regulation in Escherichia coli Promotes Pathoadaptation and Novel c-di-GMP-dependent Metabolic Capability

Figure 5

Analysis of IHE3034 bacteria for Fe(III) uptake. (a) Citrate utilization by IHE3034 and IHE3034∆ycgG2 bacteria compared to that of their ∆citT mutant derivatives. (b) Graphic representation of the local alignment of the sequence (shown in red) of the IHE3034 genome (coordinates 4927723–4959109) carrying the fecIRfecABCDE deletion against the genome of MG1655. Deletion of the operons in the IHE3034 genome is indicated with a black frame. (c) The general ability of IHE3034 and its ∆citT, ∆ycgG2 and ∆ycgG2∆citT mutants to undergo Fe(III) acquisition, as tested on a CAS plate. The yellow halo around the bacteria indicated a positive reaction for siderophore production. (d) Fe(III) citrate utilization assay (sites of inoculation marked with rings). Box I: Fe(III) citrate utilization by IHE3034 compared to that of its ∆citT mutant. Box II: Fe(III) citrate utilization by IHE3034 ∆ycgG2 compared to that of its ∆citT mutant. Box III: Reduced Fe(III) citrate utilization by wild-type IHE3034∆ycgG2 compared to that of wild-type IHE3034.

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