Figure 1 | Scientific Reports

Figure 1

From: CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells

Figure 1

The specificity of the CALHM1 antibody. (A) The specificity of the obtained CALHM1 antibody was verified by Western blotting using 40 µg of whole-cell lysates obtained from N2a cells transfected with empty vector (Mock), mouse CALHM1 (mCALHM1), mouse CALHM3 (mCALHM3), or human CALHM1 (hCALHM1). (B) Immunofluorescence confocal images of N2a cells transiently transfected with CALHM1 and 3. CALHM1 and DAPI are labeled green and blue, respectively. Scale bar, 10 µm. (C) Confocal images (green) of the circumvallate papilla immunostained with the CALHM1 antibody, immunizing peptide-preabsorbed CALHM1 antibody, or no primary antibody. (D) Immunofluorescence confocal images (green) of the circumvallate papilla of wild-type (Calhm1+/+) and CALHM1 knockout (Calhm1−/−) mice with the CALHM1 antibody. Punctate intragemmal signals were observed in Calhm1+/+ but not Calhm1−/− mice, supporting the specificity of the antibody and revealing punctate CALHM1 localization in the taste buds. Scale bar, 50 µm. Data are representative of circumvallate papillae in more than three animals.

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