Figure 2 | Scientific Reports

Figure 2

From: CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells

Figure 2

Localization of CALHM1 in the basolateral membrane of type II TBCs close to afferent nerve fibers. (A) Confocal images of double-staining of CALHM1 (green) and PLCβ2, TRPM5, or P2X2R (red) in the circumvallate taste buds. Scale bar, 20 µm. Data are representative of multiple taste buds from each circumvallate papilla in three animals. (B) Airyscan images of TBCs in the circumvallate taste buds double-stained for CALHM1 (green) and TRPM5 (red). Scale bar, 2 µm. Data are representative of five images. (C) Line plots of basolateral membrane areas carrying a single CALHM1 punctum shown in (C) (i–iii). CALHM1 signal, green line; TRPM5 signal, red line; A.U., arbitrary units.

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