Figure 4

Distinct membrane localization of CALHM and pannexin channels. (A–B) Fluorescence double-labeling of polarized MDCKII cells transfected with CALHM1-GFP and untagged CALHM3. GFP (green) and apical membrane-bound biotin (A) or Na⁺/K⁺ ATPase (B) (red) were double-stained. Biotin and Na⁺/K⁺ ATPase signals were respectively used as markers for the apical and basolateral membranes. (C) Double-staining of GFP (green) and Na⁺/K⁺ ATPase (red) in MDCKII epithelia transfected with pannexin 1-GFP. Upper panel, XY image. Lower panel, XZ image reconstructed at the blue line in its upper image. Api, apical membrane; Baso, basolateral membrane; Scale bars, 10 µm. Data are representative of four experiments.