Figure 5
Comparative proteomic analysis of HEp-2 cells treated with LG2055. (a) Antiviral activity of water-soluble and water-insoluble components fractionated from LG2055. (b) Scatterplot showing the protein expression change between LG2055 soluble fraction-treated and untreated HEp-2 cells. Proteins with a significant change with log2-fold change > 2 (upregulated) and < 2 (downregulated) are represented by red and blue lines, respectively; SRCAP is indicated in green. Proteins with no significant change by the addition of LG2055 are indicated in black. (c) Bar plot of the log2-transformed fold changes (LG2055+/untreated) of the downregulated or upregulated proteins in LG2055-treated HEp-2 cells (top and bottom, respectively). Listed proteins are indicated by their gene name. SRCAP is highlighted in green. (d) Correlation between the inhibition rate of RSV replication and the inhibition rate of SRCAP expression by treatment with the LG2055 water-soluble fraction. Data are presented as means + SD. *P < 0.05 (two-tailed Student’s t test).
