Figure 1
From: Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Expression of α-defensin in Paneth cells of enteroids. (a) DIC images of Paneth cells at the bottom of crypt-like structure in enteroid at day 3 (white arrows) acquired by using confocal laser microscopy. Scale bar: 10 μm. (b) Immunofluorescent staining of mouse α-defensin, cryptdin-1 (green) contained in the granules of Paneth cells. E-cadherin (red) as an epithelial cell marker and nuclei (blue). Left: Z-stack image of enteroid. Right: High-magnification images of Paneth cells at the bottom of crypt-like structure.
