Figure 2
From: Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Paneth cell granule secretion in response to basolateral stimuli. (a) Representative time-lapse images of Paneth cells obtained by confocal laser microscopy after adding 10 μM CCh or 100 µg/mL LPS from S. Typhimurium to the culture medium of three independent experiments for each. Real acquisition time are represented by h:mm:ss (hour:minute:second). Scale bars: 10 μm. (b) Representative area of granules (red line, top) and volume of granules (pink, bottom) in Paneth cells before and 30 min after 10 μM CCh stimulation. Scale bar: 10 μm. (c) Percent granule secretion calculated as percent area granule secretion when Paneth cells in enteroid were stimulated with 0–100 μM CCh for 30 min. 0 μM: 1.07 ± 0.33%; 0.01 μM: 5.26 ± 0.20%; 0.1 μM: 17.52 ± 0.51%; 1 μM: 54.79 ± 4.45%; 10 μM: 69.88 ± 2.56%; 100 μM: 72.49 ± 4.31%. (d) Correlation analysis between percent area granule secretion and percent volume granule secretion by 0–100 μM CCh (P < 0.0001, r = 0.90). The data were obtained from ten Paneth cells in five enteroids for each concentration. (e) Percent granule secretion of Paneth cells 30 min after adding 1–100 μg / mL LPS from S. Typhimurium to the medium. 0 µg/mL: 0.46% ± 0.25%; 1 µg/mL: 0.72 ± 0.27%; 10 µg/mL: 0.38 ± 0.26%; 100 µg/mL: 0.54% ± 0.07%. The values of (d,e) were depicted as mean ± standard error of the mean for three independent experiments. *P < 0.05, ***P < 0.0001 vs. control; n.s., not significant.
