Figure 3
From: Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Introducing reagents into enteroid lumen by microinjection. (a) Time-lapse images of fluorescein microinjection into enteroid lumen. Scale bar: 50 μm. (b) Relative Fluorescence intensities of enteroid lumen (Red ROI, 4.30 ± 0.57) and of medium (Blue ROI, 1.05 ± 0.01) at post-injection (after 5 min) against pre-injection calculated from six enteroids injected fluorescein. The values are depicted as mean ± standard error of the mean. **P < 0.001. (c) Representative Z-stack image of enteroid obtained at 180 min after adding 1 μM Rh123 into the culture medium (Pink: lumen). (d) The formula for calculating injection volume from inner diameter (r1, r2) and moving distance (h) of liquid interface of mineral oil and fluorescein in the needle at before (upper) and after (under) microinjection. Scale bar: 10 µm.
