Figure 4
From: Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Paneth cell granule secretion in response to apical LPS and S. Typhimurium stimuli. Representative time-lapse images before and after microinjection of (a) PBS, (b) 10 mg/mL LPS from S. Typhimurium and (c) 1.0 × 1010 CFU/mL S. Typhimurium ΔphoP. Scale bars: 10 μm. (d) Percent granule secretion of Paneth cells 5 min after introducing 1–10 mg/mL LPS from S. Typhimurium into enteroid lumen. PBS: 1.32 ± 0.46%, 1 mg/mL: 12.85 ± 0.90%, 10 mg/mL: 18.27 ± 2.19%. (e) Percent granule secretion of Paneth cells 20 min after introducing S. Typhimurium ΔphoP into enteroid lumen. PBS: 0.76 ± 0.75%, 1 × 1010 CFU/mL: 15.67 ± 1.74%. The values of (d,e) were depicted as mean ± standard error of the mean for three independent experiments. *P < 0.01, **P < 0.001 vs. PBS.
