Figure 5
From: Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids
Paneth cell granulogenesis after CCh-induced granule secretion. (a) Time-lapse images of Paneth cell granulogenesis obtained by confocal laser microscopy. Time denotes the elapsed time from adding CCh to the medium. Paneth cells secreted their granules by 1 μM CCh for 10 minutes, then replaced with flesh medium and follow the same Paneth cell (white arrow). The newly generated granules were presented in red arrows. Scale bars: 10 μm. (b) The time course of Paneth cell granule area relative to before 1 µM CCh stimulation. 1 µM CCh was added to the medium at time 0 followed by CCh washed out after 10 min. 0 h: 100.0%, 0.2 h: 68.2%, 1 h: 42.7%, 3 h: 42.97%, 6 h: 53.0%, 9 h: 50.7%, 12 h: 69.5%, 15 h: 75.7%, 18 h: 73.5%, 21 h: 76.7%. (c) Percent granule secretion of nine Paneth cells in five enteroids stimulated with 1 µM CCh for 10 min. 1st stimulation: 43.96 ± 7.26, 2nd stimulation (after granule replenishment): 45.89 ± 14.59. The values are depicted as mean ± standard error of the mean; n.s., not significant.
