Figure 5

Changes in the optical and chemical properties of the brain samples after tissue clearing. (A) Tissue transparency (MFP) across brain regions upon the four different clearing protocols. Scale bars represent 1 mm. (B) MFPs before and after application of each of the four tissue clearing methods. (C) Proton densities before and after application of each of the four tissue clearing methods. (D) Comparison of MFP changes between gray and white matter among the four tissue clearing methods. (E) Comparison of proton density changes between gray and white matter among the four tissue clearing methods. (F) Scatter plot of MFP versus proton density changes. Hydrogen index refers to normalized proton density with respect to the signal measured in the 1.5% agarose gel. Representative ROIs for gray matter and white matter are shown in Supplementary Fig. 4. The values used in the graphs in (C,E,F) represent means ± S.E.M.