Figure 2

Hormogonium-inducing activity of an active fraction of an ethyl acetate-soluble preparation obtained from the coralloid roots Cycas revolute. (a) Impregnation assay. (b) Sandwich assay. Two bioassays in the trials are examined by the EtOAc-soluble and the extract preliminary fractionated as active Fr-5, both as those equivalent to 2 g of coralloid roots. Approximate zones shown by circles in the impregnation assay (a) and the area shown by squares in the sandwich assay (b) are magnified as its Cy5-UV or white light. Incubation time for each assay are shown in the figure.