Figure 3
(a) Survival curves of experimental groups of larvae treated at the 4th-instar stage with 2 × 10−2 µg/mL of E. coli HT115 expressing dsCHSA_1928 lysed with chlorhexidine solution (bioinsecticide). Control groups: larvae without dsRNA addition (control), larvae with E. coli HT115 with empty PL4440 plasmid lysed with chlorhexidine (pL4440) and larvae exposed to E. coli HT115 lysed with chlorhexidine (chlorhexidine control). Relative expression of (b) CHSA and (c) CHSB in larvae treated at the 4th-instar with bacterial lysate expressing dsCHSA_1928 (pCHSA_1928), PL4440 (bacterial lysate with empty plasmid) or no dsRNA (control) (three biological replicates, three technical replicates). The RPS6 gene was used as an endogenous control to normalize the level of expression. Bars represent the means ± SEM. All asterisks indicate values significantly different from those of the controls (ANOVA followed by Tukey’s test, P < 0.05).
