Figure 4
(a) Percentages of pupal moulting and (b) adult emergence of larvae treated at the 4th-instar with 2 × 10−2 µg/mL E. coli HT115 induced by lactose expressing dsCHSA_1928 lysed with chlorhexidine solution (bioinsecticide). Control groups: larvae with no dsRNA addition (control), to E. coli HT115 lysed with chlorhexidine at a concentration of 2 × 10−2 µg/mL. and larvae exposed to E. coli HT115 with empty PL4440 plasmid lysed with chlorhexidine (pL4440). Bars represent the means ± SEM. All asterisks indicate values significantly different from those of the controls (ANOVA followed by Tukey’s test, P < 0.05). (c) Male adult insects from larvae treated at the 4th-instar with E. coli HT115 expressing dsCHSA_1928 lysed with chlorhexidine solution (bioinsecticide treatment) and not treated with dsRNA (control). Each division on the scale is equivalent to 1 mm.
