Figure 4
From: Protein SUMOylation regulates insulin secretion at multiple stages
Perturbation of SUMOylation by overexpression of the SENP1 catalytic domain inhibits insulin secretion. (A) Western blots showing SUMOylation by SUMO1 in SENP1 + SENP1-C603S expressing INS-1E cells (above), and corresponding tubulin loading control (below). (B) Insulin content of INS-1E cells infected with the indicated lentivruses, measured by ELISA (n = 20). (C) GSIS in INS-1E cells infected with control, SENP1 or SENP1-C603S virus, expressed as fold increase in insulin secretion. *p < 0.05, 1-way ANOVA (n = 8). Data are presented +/− SEM. Full, uncropped blots are shown in Supplementary Data.
