Figure 1

Interface and evaluation procedure. (a) The interface embedded in the ImageJ software with seven shortcut buttons for separate procedures of the Stripenator (1–7), an example stripe image (8) and Stripenator option window (9). The button shortcuts consist of the options (1), four buttons for testing the preferences in an example picture, such as a nucleus thresholding button (2), watershedding for nuclei (3), nucleus selection (4), thresholding of stripes (5), additionally an analyse button to run the whole analysis procedure in one or multiple images (6) and a help button (7). The Stripenator Option Window (9) offers adjustment for selection of nuclei and stripes. (b) The Stripenator workflow allows the user to analyse damaged areas and their corresponding background values by marking the damage and non-damage areas. The images show DAPI-stained nuclei in blue and γH2A.X DNA damage reference in red.