Table 2 RT-PCR & RT-qPCR gene primers used in the experiments.
From: Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function
Genes | Forward | Reverse | Product length |
|---|---|---|---|
Limk1 | CGAACAGATCCTACCTGACTCA | GAATGCTCCGTGCCACATC | 147 bp |
Limk2 | CCTACTCTGTCACGCTCATCT | GCATCCTCCACCTCCTCTAC | 229 bp |
Brn3.1 | ACCCAAATTCTCCAGCCTACAC | GGCGAGATGTGCTCAAGTAAGT | 321 bp |
β-actin | ACGGCCAGGTCATCACTATTG | AGGGGCCGGACTCATCGTA | 372 bp |
Ctbp2 | TCGGTAGTGGCTACGACAAC | CGCCGATACAGATTGAGAATGT | 133 bp |
Psd95 | GACGGGAGTGGTCAAGGTTA | GGCGAGCATAGTGAACTTCC | 120 bp |
Cofilin | CCTCTTGGTGTGGCTGTCTCTGAT | GCGCTGCCACCTAGTTTCTCTG | 467 bp |
formin | ATGGGGAACCAGGATGGGAA | CTTGCCAAGCGCCTTTTTG | 138 bp |
profilin | AACGCCTACATCGACAGCC | CGTAATGCTAACGAAGGTCTTCC | 120 bp |
tropomyosin | GTGGCTGAGAGTAAATGTGGG | TTGGTGGAATACTTGTCCGCT | 98 bp |
myosin | GCCCTCAAGGACGAGAGGT | CTCATCTCCCGTTTCTGTGAC | 197 bp |
Arp2 | TGGTGATGAGGCAAGTGAGC | ATGTGTAGTCCCACAGGTGC | 107 bp |
Arp3 | CCACGCCTGAGTTCTACCAA | TCCATCTCCCTAACCCAATCAAC | 136 bp |
TESK1 | CGAGCGTCGGAATCTCAACTTTA | ATCCCTGACCTGCTCGACT | 71 bp |
TESK2 | GGGCTGGATTACGATGCTTTC | CTTTACAGAGCCAACCAGGG | 168 bp |
Hsp25 | CAGCCTTGACCAGCCAAGAA | GAACCACTGCGACCACTCAT | 162 bp |
GAPDH | GCAAGAGAGAGGCCCTCAG | TGTGAGGGAGATGCTCAGTG | 74 bp |
