Figure 2
From: Anti-Hexokinase 1 Antibody as a Novel Serum Biomarker of a Subgroup of Diabetic Macular Edema
Identification of anti-hexokinase 1 antibody as an autoantibody in DME serum. (a) Retinal autoantigens immunoprecipitated with serum IgG of the DM group or DME group were analyzed by SDS-PAGE and silver staining. Subsequent mass spectrometry revealed that the ~102-kDa unique band (arrow in panel a) corresponded to hexokinase 1, which was confirmed by immunoblotting with rabbit monoclonal anti-hexokinase 1 antibody (b). The patients’ characteristics are shown in Supplementary Table S1. (c) Human recombinant hexokinase 1 was immunoblotted with IgG from DM or DME serum. (d) PCR analysis to determine the expression of hexokinase 1 mRNA in human retinas. (e) High levels of hexokinase 1 expression in the OPL, the outer half of the INL, and IS and, to a lesser extent, in the NFL and IPL in human retinas. Scale bar, 50 μm. GCL, ganglion cell layer; HK1, hexokinase 1; INL, inner nuclear layer; IPL, inner plexiform layer; IS, inner segment; NFL, nerve fiber layer; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment.
