Figure 4 | Scientific Reports

Figure 4

From: Quantification of hepatic steatosis in chronic liver disease using novel automated method of second harmonic generation and two-photon excited fluorescence

Figure 4

A comparison between pathologists’ assessments and the SHG Algorithm SSD. Illustrates examples of pathologist assessing hepatic steatosis using standard hematoxylin & eosin stained slides on the left and corresponding SHG algorithm derived assessment on the right. The regions with steatosis degeneration in H&E images were circled with yellow curves, and the severity of steatosis was assessed by pathologist. The portal tracts and central veins were circled with blue and red curves, respectively. In the SHG/TPEF images, the regions with steatosis degeneration were automatically labelled in blue and quantified by the algorithm. There was good correlation between the two methods of assessment.

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