Figure 4 | Scientific Reports

Figure 4

From: Isolation of photoprotective signal transduction mutants by systematic bioluminescence screening in Chlamydomonas reinhardtii

Figure 4

Photoprotective gene and protein expression in the isolated mutants under high-light conditions, including UV. (A) Genes related to photoprotection, LHCSR1, LHCSR3 and PSBS1, were analyzed with RT-PCR. The CBLP gene was used as the housekeeping control. A 100-bp DNA ladder is shown. Gel images for each gene were cropped from the same gel. (B) The LHCSR1 and LHCSR3 proteins were detected using specific antibodies against each. The ATPB protein was used as the loading control. LHCSR3-P represents LHCSR3-phosphorylated. (C) The bleaching phenotype of the mutants was visualized in a multiwell plate. The concentration was normalized to 7.0 × 106 cells/mL. Both the RNA and protein samples were collected after 1 hour, and the cultures were collected after 8 hours of irradiation under high-light with UV conditions at 300 μmol/m2/s. The samples illustrated here are representative of three biological replicates.

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