Figure 2
From: Identification of Streptococcus pneumoniae by a real-time PCR assay targeting SP2020
Genomic comparison between regions of interest in TIGR4 (NCBI accession number AE 005672.3) and strains (pneumococci and non-pneumococci) with atypical results when tested for the presence of lytA, piaB, or SP2020: (a) serotype 6B pneumococcal strain (ATCC BAA-342) testing negative for piaB; (b) S. pseudopneumoniae strains Spain2270 and Spain9880 testing positive for lytA; (c) S. pseudopneumoniae strains EL2652N1 and Spain939 testing positive for piaB; (d) S. pseudopneumoniae/S. mitis strain Spain3473 testing positive for SP2020. Regions with nucleotide identity ≥93% are represented either in red (same orientation) or in blue (reverse orientation). Regions highlighted in yellow indicate the gene of interest. All comparisons were performed by Double ACT v2 and visualized using Artemis Comparison Tool (ACT) release 17.0.1.
