Table 2 Properties of non-typeable S. pneumoniae and other strains with unusual results when tested by real-time PCR for lytA, piaB, and SP2020.

From: Identification of Streptococcus pneumoniae by a real-time PCR assay targeting SP2020

Strain

Species classificationa (serotype)

Real-time PCR (CT)b

MLST allelic profilec

ST

lytA

piaB

SP2020

aroE

ddl

gdh

gki

recP

spi

xpt

DCC1365

S. pneumoniae (NT)

21

37

21

8

53

37

9

29

2

12

344

DCC635

S. pneumoniae (NT)

23

NA

23

8

53

37

9

29

2

12

344

DCC646

S. pneumoniae (NT)

23

NA

21

8

53

37

9

29

2

12

344

PT526b

S. pneumoniae (NT)

23

39

23

8

53

37

9

29

2

12

344

PT1493

S. pneumoniae (NT)

20

24

21

2

164

13

2

29

91

19

1617

PT1683

S. pneumoniae (NT)

21

22

21

7

1

11

10

1

6

8

156

PT1718

S. pneumoniae (NT)

22

NA

22

70

59

10

15

16

2

105

1540

PT1804b

S. pneumoniae (NT)

22

NA

22

8

26

74

19

15

6

40

888

PT2293b

S. pneumoniae (NT)

21

NA

21

8

53

37

9

29

2

12

344

PT3201

S. pneumoniae (NT)

21

26

22

2

141

13

2

29

91

19

1153

PT4014

S. pneumoniae (NT)

20

26

22

2

141

13

2

29

91

19

1153

PT4222

S. pneumoniae (NT)

20

26

21

2

59

13

2

29

91

19

1156

WL1084

S. pneumoniae (NT)

21

37

23

8

71

5

2

27

2

11

448

WL1514

S. pneumoniae (NT)

23

NA

25

8

53

37

9

29

2

12

344

ATCC BAA-342

S. pneumoniae (6B)

20

NA

22

7

67

6

9

2

6

1

384

EL2652N1

S. pseudopneumoniae

NA

24

NA

103

656

477 (98)

250 (97)

264 (97)

313 (99)

595 (95)

Spain939

S. pseudopneumoniae

NA

26

NA

427

751 (97)

492

345 (99)

139 (98)

442 (99)

105 (99)

Spain2270

S. pseudopneumoniae

24

NA

NA

427

447 (97)

381 (98)

197 (94)

373

442 (99)

735 (96)

Spain9880

S. pseudopneumoniae

24

NA

NA

427

447 (97)

381 (98)

197 (94)

373

442 (99)

735 (96)

Spain3473

S. pseudopneumoniae/S.mitis

NA

NA

23

427

751 (97)

40

382 (99)

29

442 (99)

105 (99)

  1. aSpecies classification was done based on MLST/MLSA as described in Materials and Methods section. bNA, no amplification in 45 cycles. cMLST allelic profile of non-pneumococcal strains indicates the allele number of the closest match; the similarity (in %) is indicated in parenthesis. S. pneumoniae MLST database was last accessed on February 8, 2018.