Figure 5 | Scientific Reports

Figure 5

From: Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

Figure 5

Schematic illustration of basic molecular characterization of general odorant binding protein1 (GOBP1) from Spoladea recurvalis (a) cDNA and derived amino acid sequence of Spre-GOBP1.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. (b) qRT-PCR analysis shown the expression of Spre-GOBP1 relative to each development stages.(Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day).GAPDH was used as an internal control. Bar represents ±standard error. Different letters (a,b,c) shows the significant differences (P < 0.05). (c) Maximum Likelihood tree was constructed based on general odorant–binding protein 1 the amino acid sequence using MEGA 6.0 with default settings model LG + G inferred from 1000 replicates.

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