Figure 6

Schematic illustration of basic molecular characterization of pheromone binding protein (PBP) from Spoladea recurvalis. (a) 5′ missing partial cDNA and derived amino acid sequence of Spre-PBP. The conserved cysteine residues are marked in square box. (b) qRT-PCR analysis shown the expression of Spre-PBP relative to each development stages.(Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters (a,b,c) shows the significant differences (P < 0.05). (c) Maximum Likelihood tree was constructed based on Pheromone binding protein the amino acid sequence using MEGA 6.0 with default settings model LG + G + I inferred from 1000 replicates.