Figure 5
HB-GRKO LMC and HB-GRKO present with severely disrupted adrenals. (A) Analysis of male adrenal weight revealed an increase in weight in both HB-GRKO LMC and HB-GRKO mice compared to BL6 controls (One-way ANOVA; n = 8, ***p < 0.0001, **p < 0.001, error bars SEM). (B) Analysis of female adrenal weight revealed no differences between any of the cohorts and WT BL6 controls. (C) Morphology analysis of male HB-GRKO LMC and HB-GRKO adrenals both displayed major disruption to the entire cortex. Due to the disruption observed in littermate controls, an external WT BL6 cohort was included in the analysis. Enlarged images of regions of interest denoted by squares. (D) Morphology analysis of female HB-GRKO LMC and HB-GRKO adrenals both displayed major disruption to the entire cortex, with abnormal X-zone morphology. Enlarged images of regions of interest denoted by squares. (E) Circulating corticosterone levels are elevated in both HB-GRKO LMC and HB-GRKO males compared to WT BL6 (one-way ANOVA; n = 8, *p < 0.05, *p < 0.05, error bars SEM). (F) Circulating corticosterone levels do not change in HB-GRKO LMC and HB-GRKO females compared to BL6 controls. Abbreviations; ZG = zona glomerulosa, ZF = zona fasciculata, X = X-zone. Scale bars 100 µm.
