Figure 7
HB-GRKO mice show aberrant apoptosis throughout the adrenal cortex. Cell clearance from the cortex occurs at the cortex-medulla boundary which can be observed in WT Bl6 male and female controls via immunohistochemical analysis. HB-GRKO female Cleaved Caspase 3 protein localisation show abundant positive cells throughout the cortex. Inserts denoted by squares. (B) Cell counts show no changes between any of the male cohorts analysed. (C) Cleaved Caspase 3 counts reveals a significant increase in cell death throughout the cortex (One-way ANOVA; n = 5, ***p < 0.0001, ****p < 0.0001, Tukey’s post-hoc analysis, error bars SEM), consistent with the immunohistochemistry analysis. Scale bars 50 µm. Abbreviations; ZG = zona glomerulosa, ZF = zona fasciculata. Scale bars 100 µm.
