Figure 3
From: HIV infection and latency induce a unique metabolic signature in human macrophages
HIV infection did not alter glycolysis. Uninfected (UI) and HIV infected cultures (HIV) at different stages were subjected to ECAR, extracellular acidification rate measurement, and Seahorse analysis to examine glycolysis. We injected glucose (G), oligomycin (O), and 2-deoxy-D-glucose (2DG). Treatment with glucose increases ECAR, while subsequent injection with oligomycin forces cells to use only lactic acid fermentation for ATP production and further increases ECAR. Inhibition with 2DG completely shuts down any ATP production from glycolysis and thus reduces ECAR. (A) Changes in ECAR during early stages; (B) mid-stages, and (C) late stages. (D) Corresponds to an amplification of the HIV infected curve shown in C. (E) Corresponds to the % change in ECAR in response to oligomycin during all stages of infection. No significant changes were observed. (F) Represents the blocking stage of 2DG.
