Figure 4
From: HIV infection and latency induce a unique metabolic signature in human macrophages
HIV infection increased lipid accumulation and interaction of lipids with the mitochondria. Cells were fixed with 4% PFA but not permeabilized to prevent lipid loss. Next, cells were stained with DAPI, BODIPY, and phalloidin to label nuclei, lipids, and actin, respectively. (A) A representative example of uninfected (UI) and HIV infected (HIV) macrophage cultures staining used to measure lipid production at the late stage of infection. (B) Quantification of lipid staining intensity indicates that HIV fused cells had a higher amount of lipids as compared to uninfected cells (UI) or HIV unfused cells (HIV single). *p = 0.0230, n = 6. (C) Quantification of lipids per nucleus was normalized to the number of nuclei per cell, with no significant difference per nucleus between uninfected cultures and HIV infected cultures. (D,E) Showing two different examples of proliferating and fused mitochondria (M) and their close interaction with lipid droplets (LD), see the arrow.
