Figure 1
From: Methodological refinement of Aldara-induced psoriasiform dermatitis model in mice
Comparison of functional parameters in Aldara-induced psoriasiform dermatitis using original and modified protocols. (a) Clinical signs of topical Aldara treatment on C57BL/6 mice throughout the 5 day experiment. 62.5 mg vaseline (OP group - Vaseline) or Aldara (OP group - Aldara) was applied to the back skin of mice. 25–25 mg vaseline (V) or Aldara (A) was applied to the shaved back skin of animals using Finn chambers (MP group). (b) Representative images of blood perfusion changes on C57BL/6 mice dorsal skin induced by topical application of vaseline or Aldara in the OP group and in the MP group using Finn chambers. (c) Percent increase of back skin thickness after vaseline or Aldara treatment in OP or MP groups compared to day 0 baseline values. Data are mean ± SEM for n = 15/group. ***p < 0.001 vaseline vs. Aldara-treated sites, ###p < 0.001 Aldara-treated OP group vs. Aldara-treated MP group, based on repeated measures 2-way ANOVA followed by Bonferroni’s post hoc test. (d) Percent change of blood perfusion in dorsal skin after vaseline or Aldara treatment compared to the day 0 values. Data are mean ± SEM for n = 15/group. *p < 0.05; **p < 0.01 vaseline vs. Aldara-treated sites, based on repeated measures 2-way ANOVA followed by Bonferroni’s post hoc test. (e) Skin scaling scores after vaseline of Aldara treatment in OP group or MP group. Scaling was graded from 0 to 4 (0: absent to 4: severe). Data are mean ± SEM for n = 7–15/group. ***p < 0.001 vaseline vs. Aldara-treated sites, based on 2-way ANOVA followed by Bonferroni’s post hoc test. OP: original protocol, MP: modified protocol.
