Figure 4

Modified protocol reduced the systemic effect of IMQ treatment in C57BL/6 mice. (a) Percent change of body weight after vaseline or Aldara treatment using two different methods. Data were expressed as percent change of body weight compared with day 0 baseline initial values. Data are mean ± SEM for n = 15/group. ^#p < 0.05; ^##p < 0.01; ^###p < 0.001 Aldara-treated OP group vs. Aldara-treated MP group, ***p < 0.001 vaseline vs. Aldara-treated group, based on 2-way ANOVA followed by Bonferroni’s post hoc test. (b) Effect of the two different disease induction techniques for the development of the splenomegaly. Spleens were collected and weighted at the end of the experiment. Data are mean ± SEM for n = 15/group. **p < 0.01; ***p < 0.001, based on one-way ANOVA followed by Bonferroni’s post hoc test. OP: original protocol, MP: modified protocol.