Figure 1
From: Identification of Novel Inhibitors of DLK Palmitoylation and Signaling by High Content Screening
Palmitoylation-dependent localization of DLK-GFP to the Golgi apparatus in HEK293T cells. (A) HEK293T cells were transfected to express wild type DLK-GFP and subsequently fixed. DLK-GFP and the Golgi marker GM130 were detected with specific antibodies and nuclei were detected using the DNA dye DAPI. (B) HEK293T cells were transfected as in A to express either wild type DLK-GFP (DLK-GFP) or a DLK palmitoylation site mutant (C127S-DLK-GFP). C127S mutation, or treatment with the palmitoylation inhibitor 2BP, diffuses the Golgi-associated clusters of DLK-GFP.
