Figure 4
Validation of predicted novel circRNA in different tissues. (A–E) PCR based confirmation of circRNA junctions in (A) Blood, (B) Brain, (C) Muscle, (D) Heart and (E) Gills, respectively. Divergent primers facing outwards were used to amplify cDNA (C), DNA (D) and negative control (NC). Convergent primers for actb1 gene used in every tissue as control. L- 100 bp Ladder (Fermentas, USA).
