Figure 6
Quantitative validation of putative novel circular RNAs across different tissues. qRT-PCR based relative expression analysis of candidate circRNAs in (A). Blood, (B) Brain, (C) Muscle, (D) Heart, (E) Gills. The expression levels of linear transcripts of parent gene of each candidate circRNA were also estimated. Tissue specific protein coding gene marker tal1(blood), mdka(brain), tnnt2c(muscle), vmhc(heart) and cx44.2(gills) were used as controls. Data was normalized with the expression levels of actb1. Data was collected from independent experiments and represented as relative mean fold change ± SD across tissues. circRNAs examined in a particular tissue type displayed relatively high expression in the specific tissue when compared to other tissue types.
