Figure 3
Comparison of the total summed relative abundance of each lipid class between the control and treatment (2-DG, COR, and 2-DG + COR) groups on human melanocytes (HEMn-LP) and melanoma cells (A375 and A2058). The relative abundance of lipids in the cells was quantified by comparing the intensities of lipid ion peaks with the intensity of the internal standard and subsequent normalization of quantified ion abundances with protein equivalents used for cellular lipid profiling. Each bar on the graph represents the sum of the protein-adjusted normalized lipid abundance for all the identified lipid species within each lipid class, and the error bars indicate the standard deviation of triplicate experiments. The asterisk (*) indicates significant differences compared with the control group, as determined using Student’s t-test (p < 0.05). (A) Cer, ceramide; (B) PE, phosphatidylethanolamine; (C) PG, phosphatidylglycerol; (D) PS, phosphatidylserine; (E) PI, phosphatidylinositol; (F) PC, phosphatidylcholine; and (G) TG, triacylglycerol.
