Figure 4

ABX treatment in the context of BDPP effects OTUs association with phenolic acids plasma concentration. (a) Quantitle-quantile plot comparing the observed p-value of fecal OTUs proportion collected on Day 21 (x-axis) vs. BDPP-driven phenolic acid plasma concentration collected on Day 22 (y-axis) against expected p-value under null hypothesis (i.e., no associations) in SD + BDPP, n = 9 vs. SD + ABX + BDPP, n = 17) mice groups by plotting their quantiles against each other. Points that lie approximately on the line y = x represent similar distributions. Points above the line y = x represent observed p-values that are more significant than expected. (b) Heat map visualizing the inferred influence of the specific OTUs on behavior traits through modifying plasma phenolic acids’ concentration. Red, OTUs positively associate with freezing percent through positive association with the particular phenolic acid; Orange, OTUs negatively associate with freezing percent through negative association with the particular phenolic acid; Blue, OTUs positively associate with freezing percent through negative association with the particular phenolic acid; Green, OTUs negatively associate with freezing percent through positive association with the particular phenolic acid; White, no significant influence of OTU on freezing percent detected.