Figure 2

Temporal variations in SOX levels in (a) HCT 116 wt cells and (b) HCT 116 p53−/− cells for untreated control, 3 µM, 9 µM and 15 µM menadione (MD) treatment. The cells were seeded in 6 well plates and synchronized for 24 h in serum free medium. 0 h corresponds to the time of medium change to DMEM with 10% FBS with (treated) or without (untreated) MD. The time where SOX levels peak are different for different concentrations of MD in the wt cells whereas the trend is unchanged in the p53−/− cells. The values are expressed as mean ± SD, n = 3. The data was shown to be significant using two way ANOVA with p < 0.001.