Figure 3
From: A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation
Location of the m6A motifs in the 3′ sites of the (A) SOCS1 and (B) SOCS3 genes. RNA immunoprecipitation of m6A followed by RT-QPCR for the 3′ sites of (C) SOCS1 and (D) SOCS3. Data are represented as the mean and standard error of four independent experiments. p-value *p < 0.05. Validation of the m6A-RT-QPCR method for the relative quantification of m6A motifs in SOCS1 and SOCS3 genes in RNA extracted from (E) HCT15 and (F) Caco2 cells. Data are represented as the mean and standard error of three independent experiments. **p-value < 0.01, *p < 0.05; based on paired Student’s t-test.
