Figure 3 | Scientific Reports

Figure 3

From: Targeted delivery of TLR3 agonist to tumor cells with single chain antibody fragment-conjugated nanoparticles induces type I-interferon response and apoptosis

Figure 3

Generation of tumor-targeting scFv(h-AM1)-BAP-RICIA immunoconjugates. (a) Titration of scFv(h-AM1)-BAP against avidin in increasing molar ratios for the estimation of adequate conjugation conditions. Western blot analysis was performed to detect complexes via the c-myc epitope and free biotinylated scFv(h-AM1)-BAP. (b) Schematic representation of the successive conjugation of tumor-targeting RICIA. (c) Size of assembled scFv(h-AM1)-BAP-RICIA immunoconjugates was investigated using in situ atomic force microscopy (AFM).

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