Figure 4
From: Selective oxidation of B800 bacteriochlorophyll a in photosynthetic light-harvesting protein LH2
HPLC chromatograms of chlorophyllous pigments in native LH2 (A) and oxidized LH2 (B,C). Chromatograms (A–C) were recorded at 770, 770, and 677 nm, respectively. LH2 proteins were directly injected and eluted on a reverse-phase column 5C18-AR-II (6 mm i.d. × 250 mm) with a guard column 5C18-AR-II (4.6 mm i.d. × 10 mm) with methanol at the flow rate of 1.0 mL min–1. Signals denoted by × were due to direct injection of LH2 solutions.
