Figure 2
The specific T cells response by IFN-γ ELISPOT after peptides immunization. Thirty days after the last immunization in mice, the lung and splenocytes were isolated and stimulated with HKRB51 (Heat killed RB51). SAG4 peptide and PBS were used as negative controls. IFN-γ producing T cells were detected by ELISPOT assay. (A) Number of IFN-γ producing T cells. One-way ANOVA was used for the Statistical Analysis. Data are shown as mean ± SD. **P < 0.01. *P < 0.05. SFU, spot-forming units. (B) Representative ELISPOT results.
