Figure 4
The process of immunization and B. melitensis infection. Mice were immunized intranasally (i.n, 15 μL per nostril) with PBS (Control group), 30 μg T epitopes (T epitope group), B epitopes (B epitope group) and TB epitopes (TB epitope group), respectively. Enhanced immunization was performed on the 15th, 30th and 45th day after the primary immunization, respectively. On the 75th day after the first immunization, the mice were intranasally infected with 5 × 105 CFU Brucella strain 16 M. Fourteen days after nasal infection, mice were sacrificed after anesthesia. The lung and spleen of mice were isolated for subsequent studies.
