Figure 5
PC-mediated increase of NO within the media might be responsible for the PC-mediated VEGF-A gene expression. (A) A schematic figure describing three different cell treatment methods with PC. (B) At 6 hours post-treatments of cells by three different PC treatment methods, total RNA was extracted and used for RT-PCR analysis. Data shown are the representatives of three independent experiments, *p < 0.005. DT: direct treatment; IDT: indirect treatment; DT-MC: direct treatment and media change. (C) The concentration of NO2− level in the cell growth media was detected. Cell growth media (2 ml) was treated with PC for 5 minutes to make plasma-activated media (PAM) and then immediately used for a Griess assay. Data shown are the average of three independent experiments, *p < 0.0005.
