Figure 6
From: RNA Stabilizes Transcription-Dependent Chromatin Loops Induced By Nuclear Hormones
E2-induction changes the 5′ end of the CAV1 loop and determines the ends of the short CAV1 transcript. Schematic diagram of the CAV1 promoter-ERE-polyA loops, represented as a single loop, in cells exposed to E2. In the absence of E2, the loop at the 5′ end (promoter-ERE) was not detectable. The CAV1 transcript synthesized under these conditions is indicated as a long transcript originating from Start 1 (Figs 3A and 4A). Loop modification upon E2-induction. E2 induces two RNAs corresponding to oligonucleotide 10 and 7 (Fig. 5D) located upstream of Start 2 and at the polyA site, respectively, which conceivably influence this modification. The loop ERE-PolyA, described in Fig. 2D, is now detectable and is sensitive to RNAse H1. The CAV1 transcript synthesized under these conditions is indicated as short transcript originating from Start 2 (Figs 3A and 5).
