Figure 1
From: Nucleation and Formation of a Primary Clot in Insect Blood
Growth and shrinkage of cellular aggregates in incubating hemolymph. (A–E) A gallery of bright-field micrographs of an in-vitro incubating droplet of blood from a larva of M. sexta. Cells can be seen as bright and dark circles freely floating in the fluid (grey background) and as part of the aggregate (blue outline); t is the incubation time of the sample. The boundary of the aggregate was determined using a Photoshop user-assisted image analysis algorithm, Quick Selection Tool. (F) Average non-dimensional area of aggregates of three larvae as a function of incubation time, t. The area was normalized by its maximum for each aggregate. The rate of area shrinkage approaches 0 after about 10 minutes of incubation.
