Figure 5

Heat-shock induced bam can only partially rescue the phenotypic defects induced by HP1 knockdown. (a,b) Staining for Vasa (green) and DNA (red) on whole mounts adult ovaries from nos-G4 > HP1^RNAi (a) and nos-G4 > HP1^RNAi; hs-bam (b) females. (b) The dashed white box in the left panel is magnified in the right panel. (c) Quantification of ovarioles containing developing egg chambers in heat shocked HP1 depleted females carrying or not the P[hs-bam] transgene (625 and 984 ovarioles scored for nos-G4 > HP1^RNAi and nos-G4 > HP1^RNAi; hs-bam, respectively). Bar graph represents the mean ± SEM from five indipendent experiments. Statistical significance was determined by unpaired t test (****p < 0.0001). (d) Staining for Spectrin (green) and DAPI (red) on germaria obtained from bam-G4 > HP1^RNAi females.