Figure 2

Genetic analysis of liver triglycerides content in 129S6xBALB/c F2 mice fed high fat diet. Concentration of liver triglycerides was determined in BALB/c and 129S6 mice fed control carbohydrate diet (CHD) or high fat diet (HFD) and in 129S6xBALB/c F2 mice fed HFD (A). Distribution of liver triglycerides in F2 mice shows the strong variability of the phenotype in the cross (B). Solid black line indicates phenotype means in the F2 cohort, and green and red arrows indicate mean values of corresponding phenotypes in 129S6 and BALB/c mice, respectively. Genome-wide scans for liver triglycerides in HFD fed 129S6xBLAB/c F2 mice identified quantitative trait loci of marginal significance on chromosomes 4 and 18 (C). LOD scores are plotted against map distances (centimorgans). Effects of genotypes at marker loci rs3702881 (chromosome 4) and rs13483370 (chromosome 18) exhibiting the strongest evidence of linkage to liver triglycerides are illustrated by mean values (±SEM) of the phenotypes calculated according to the genotype homozygous for the 129S6 or BALB/c alleles, or heterozygous at the loci. *P = 0.001 statistically different between 129S6 fed HFD or CHD; ^$ P = 0.006 statistically different between fat fed 129S6 and fat fed BALB/c.