Figure 4
From: Unexpected genomic rearrangements at targeted loci associated with CRISPR/Cas9-mediated knock-in
Cas9n technology induces unexpected rearrangements at the targeted locus. (A) Number of experiments and loci targeted in mouse ES cells using Cas9n technology and cHR, tested by Southern Blot (SB) with an external probe (see Sup Table for further details). (B) Blot of Cas9n experiment #34 targeting locus E showing profiles obtained with an external probe after EcoNI digestion (WT: 4011; Rec: 7108). Note that clones 34b, 34 f, 34i, 34 l and 34n show bands of unexpected size. (C) Box plots of percentages of clones showing bands of unexpected sizes by SB with Cas9n and cHR for all loci. L: ladder.
