Table 2 Oligonucleotides used in this study.

From: Microbial Degradation of Nicotinamide by a Strain Alcaligenes sp. P156

Name

Sequence (5′-3′) (restriction sites are underlined)

naaA-F

CGCGGATCCATGGCTATTCAGATTGAT

naaA-R

CCGCTCGAGTTATTGTCTGGATTTCTG

naaD-F

CGCGGATCCATGGCAGTTAGTGATTATC

naaD-R

CCGCTCGAGTCATTCGTACTCACCAACG

naaE-F

CGCGGATCCATGAGTACCTTTCTTTACG

naaE-R

CCGCTCGAGTCACACCAGGCGTTGGCCC

naaF-F

CGCGGATCCTTGGAGCCTAAAGCTCCT

naaF-R

CCGCTCGAGTTAAACTCCAATCAACTT

  1. PCR primers naaA-F/-R, naaD-F/-R, naaE-F/-R and naaF-F/-R are used for the construction of expression recombination plasmid pET-28a-naaA, pET28a-naaD, pET28a-naaE, and pET28a-naaF.
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