Figure 4 | Scientific Reports

Figure 4

From: The harmful effects of acute PM2.5 exposure to the heart and a novel preventive and therapeutic function of CEOs

Figure 4The alternative text for this image may have been generated using AI.

PM2.5 induced the overload of intracelluar Ca2+ and abnormal abundances of calmodulin in cardiac tissue. The level of intracellular Ca2+ was determined by flow cytometry. RYR2 and SERCA2a mRNA levels were assayed by real-time RT-PCR using the 2−ΔCt method. RYR2 protein level was detected by WB and IHC. PM2.5 increased the level of intracellular Ca2+ (A,B) and RYR2 (D–G), and reduced the expression of SERCA2a (C). Data are expressed as mean ± standard error of the mean (n = 3–5, **P < 0.01, *P < 0.05 versus the control group).

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