Figure 7

CEOs suppressed PM2.5-induced oxidative stress. HO-1 and SOD1 mRNA levels were assayed by real-time RT-PCR using the 2−ΔCt method. HO-1 protein level was detected by WB and IHC. CEOs reduced the levels of HO-1 (B–E) and increased the expression of SOD1 (A). Data are expressed as mean ± standard error of the mean (n = 3–5, **P < 0.01, *P < 0.05 versus the PM2.5 + saline group I).